Duke University

PTH225

Introduction to Systemic Histology

send questions or comments to Laura P. Hale, MD. PhD. at hale0004@mc.duke.edu

 

The purpose of this course is to teach students how to identify various cell types in standard histologic sections and to identify a variety of normal tissues. This course will be useful to any student whose research requires examination of tissue sections. Structure/function relationships will be emphasized, using an organ system approach. The scheduled class time will include lecture and demonstration of various tissue slides by the instrutor. Beginning in 2005, the course will be taught primarily using "virtual microscopy", supplemented by examination of glass slides using a conventioanl optical microscope. In virtual microscopy, glass slides scanned at high resolution are viewed on a computer monitor. Students have the ability to select regions of intrest for higher magnification viewing and in many cases, can also change the focus. Unless arrangements are made in advance, students are expected to bring their own laptop computer to class for each class period.

Text: Histology: A Text and Atlas (4th Edition), by M.H. Ross, G.I. Kaye, W. Pawlina Lippincolt. Williams and Wilkins, Philadelphia, 2003. (Available at the Medical Center Bookstore $59)

 

Accessory Gastrointestinal Glands

Image 1: Serous demilunes from submandibular salivary gland, H&E, x100

Image 2: Pancreatic islet of Langerhans, glucagon immunoperoxidase stain, x66

Image 3: Pancreatic islet of Langerhans, insulin immunoperoxidase stain, x66

Image 4: Pancreatic islet of Langerhans, somatostatin immunoperoxidase stain, x66

Image 5: Mucous acini from labial (minor) salivary gland, H&E, x50

Image 6: Mixed (serous and mucous) acini from submandibular salivary gland, H&E, x100

Image 7: Exocrine pancreas, H&E, x66

Image 8: Parotid salivary gland, H&E, x100

Image 9: Serous acini from parotid salivary gland, H&E, x100

Image 10: Excretory duct from submandibular salivary gland, H&E, x50

Image 11: Submandibular salivary gland, H&E, x100

Image 12: Serous demilunes from submandibular salivary gland, H&E, x100

Image 13: Submandibular salivary gland, H&E, x100

Image 14: Pancreas, with islet of Langerhans, H&E, x66

Image 15: Liver, H&E, x33

Image 16: Pancreas, with islet of Langerhans, H&E, x66

Image 17: Pancreas, with portions of 3 islets of Langerhans, H&E, x66

Image 18: Submandibular gland, H&E, x66

 

Endocrine Organs (images 33-51 provided by Edward Bossen, M.D.)

Image 30: Thyroid, H&E, x66

Image 31: Thyroid, H&E, x66

Image 32: Adrenal gland, H&E, x13

Image 33: Adrenal gland, H&E, x4

Image 34: Adrenal gland, cortex and medulla, H&E, x13

Image 35: Adrenal gland, zona glomerulosa of cortex, H&E, x66

Image 36: Adrenal gland, cortex, H&E, x33

Image 37: Adrenal gland, zona reticularis of cortex, H&E, x66

Image 38: Adrenal gland, medulla, H&E, x66

Image 39: Adrenal gland, neurons present in adrenal medulla, H&E, x66

Image 40: Parathyroid gland, H&E, x13

Image 41: Parathyroid gland, chief and oxyphil cells, H&E, x400

Image 42: Parathyroid gland, chief and oxyphil cells, H&E, x400

Image 43: Pituitary gland, colloid area, H&E, x66

Image 44: Pituitary gland, prolactin immunoperoxidase stain (brown), x132

Image 45: Pituitary gland, ACTH immunoperoxidase stain (brown), x132

Image 46: Posterior pituitary (neurohypophysis), H&E, x66

Image 47: Pituitary gland, anterior and posterior portions, H&E, x66

Image 48: Anterior pituitary, basophils, acidophils, and chromophobes, H&E, x132

Image 49: Anterior pituitary, H&E, x66

Image 50: Anterior pituitary, human growth hormone (HGH) immunoperoxidase stain, x132

Image 51: Anterior pituitary, synaptophysin immunoperoxidase stain (brown), x66

 

Connective Tissue, Bone, Muscle

Image 60: Brown and white adipose tissue, periadrenal region, H&E, x66

Image 61: Smooth muscle, muscularis externa of colon, H&E, x132

Image 62: Brown adipose tissue, periadrenal region, H&E, x132

Image 63: White adipose tissue, periadrenal region, H&E, x132

Image 64: Smooth muscle, muscularis externa of colon. H&E, x132

Image 65: Hyaline cartilage, bronchus, H&E, x66

Image 66: Bone, vertebral column, H&E, x66

Image 67: Tendon, dense regular connective tissue, H&E, x66

Image 68: Skeletal muscle, longitudinal section, H&E, x132

Image 69: Smooth muscle, myometrium of uterus, H&E, x132

 

Hematopoietic and Lymphoid Systems

Image 100: Spleen, H&E, x3.3

Image 101: Spleen, periarteriolar lymphoid sheath, H&E, x33

Image 102: Adenoids, H&E, x132

Image 103: Thymus, from infant, H&E, x66

Image 104: Plasma cell, Wright stain, x400

Image 105: Bone marrow, H&E, x66

Image 106: 7 week fetus (to show location of thymus), H&E, x3.3

Image 107: Thymus, from 7 week fetus, H&E, x33

Image 108: Thymus, from 38 year old female, H&E, x16

Image 109: Thymus, from 42 year old male, H&E, x3.3

Image110: High endothelial (post-capillary) venule, from adenoid tissue, H&E, x132

Image 111: Adenoids, H&E, x66

Image 112: Lymph node, H&E, x16

Image 113: Spleen, red pulp, H&E, x66

Image 114: Tonsil, H&E, x33

Image 115: High endothelial (post-capillary) venules, from tonsil, H&E, x132

Image 116: Mast cells, transmission electron microscopy

Image 117: Mast cell, safranin stain, x400

Image 118: Mast cell, scanning (left) and transmission (right) electron microscopy

Image 119: Mast cell cluster, toluidine blue stain, x400

Image 120: Mast cell cluster, toluidine blue stain, x400

Image 121: Mast cell, safranin stain, x400

Image 122: Mast cell, toluidine blue, x400

Image 123: Peyer's patch, ileum, H&E, x33

 

Gastrointestinal System

Image 200: Duodenum: mucosa, muscularis mucosae, and submucosa, H&E, x66

Image 201: Colon, H&E, x66

Image 202: Myenteric plexus of colon, H&E, x132

Image 203: Muscularis externa of colon, H&E, x13

Image 204: Myenteric plexus of colon, H&E, x132

Image 205: Stratified squamous epithelium, esophagus, H&E, x33

Image 206: Stomach, gastrin immunoperoxidase stain (brown), x33

Image 207: Stomach, simple columnar epithelium, H&E, x50

Image 208: Ileum, H&E, x3

Image 209: Ileum, H&E, x132. Arrows point out brush border on epithelial cells.

Image 210: Colon: mucosa, submucosa, muscularis externa, serosa, H&E, x4

Image 211: Colon, H&E, x33

Image 212: Ileum, H&E, x33

Image 213: Appendix, H&E, x3

Image 214: Appendix, H&E, x3

Image 215: Oral mucosa, H&E, x33. Note salivary gland underlying non-keratinized stratified squamous epithelium.

 

Female Reproductive System

Image 300: Breast, H&E, x66

Image 301: Breast, H&E, x4

Image 302: Placenta, chorionic villus, H&E, x132

Image 303: Endometrium, late secretory (day 24), H&E, x13/ x33

Image 304: Endometrium, proliferative phase, H&E, x13

Image 305: Endometrium, secretory phase, H&E, x13

Image 306: Fallopian tube, ciliated columnar epithelium, H&E, x400

Image 307: Lactating breast, H&E, x13

Image 308: Lactating breast, H&E, x66

Image 309: Cervix, stratified squamous epithelium, H&E, x50

Image 310: Endocervix, ciliated columnar epithelium, H&E, x50

Image 311: Breast, H&E, x13

Image 312: Chorioamnionic membrane, H&E, x33

Image 313: Placenta, chorionic villi, H&E, x33

Image 314: Placenta, chorionic villi, H&E, x66

Image 315: Fallopian tube, showing variation in size and complexity along tube length, H&E, x1

Image 316: Ovary, simple cuboidal germinal epithelium, H&E, x132

Image 317: Ovary, germinal epithelium and tunica albuginea, H&E, x66

Image 318: Ovary, cortical stroma with multiple primordial follicles, H&E, x33

Image 319: Ovary, medulla containing multiple blood vessels, H&E, x33

Image 320: Fallopian tube, columnar epithelium, H&E, x132

Image 321: Fallopian tube, H&E, x4. Note vessels in mesosalpinx at left.

Image 322: Fallopian tube, H&E, x13

Image 323: Mesothelium overlying fallopian tube, H&E, x66

 

Cardiovascular System

Image 400: Heart, H&E, x130

Image 401: Artery and vein, periadrenal region, H&E, x66

Image 402: Artery, H&E, x13

Image 403: Artery, H&E, x25

Image 404: Artery, H&E, x100

Image 405: Heart, H&E, x33

Image 406: Artery, H&E, x33. Arrow points to internal elastic lamina.

Image 407: Vein, H&E, x33. N denotes nerve adjacent to vessel.

 

Respiratory System

Image 450: Bronchial wall, cartilage and mucous glands, H&E, x33

Image 451: Bronchial epithelium, H&E, x132

Image 452: Bronchial epithelium, H&E, x132

Image 453: Bronchial epithelium. H&E, x132

 

Skin and Associated Structures (images 500 - 518 provided by Christopher Shea, M.D.)

Image 500: Epidermis, H&E, x100

Image 501: Epidermis, H&E, x200

Image 502: Epidermis (note prominent melanocyte), H&E, x200

Image 503: Skin, showing papillary and reticular dermis and vascular plexus, H&E, x20

Image 504: Dermal blood vessels and nerve, H&E, x200

Image 505: Dermal artery, H&E, x50

Image 506: Dermal vein, H&E, x50

Image 507: Artery and nerve, at junction of reticular dermis and subcutaneous adipose tissue, H&E, x40

Image 508: Skin, showing epidermis, dermis, and eccrine unit. H&E, x5

Image 509: Eccrine gland, H&E, x200

Image 510: Epidermis, showing eccrine duct exiting to skin surface (acrosyringium), H&E, x100

Image 511: Base of hair follicle (bulb), H&E, x50

Image 512: Middle of hair follicle (isthmus), including sebaceous gland. H&E, x50

Image 513: Upper portion of hair follicle (infundibulum), H&E, x50

Image 514: Smooth muscle associated with hair (arrector pili muscle), H&E, x200

Image 515: Sebaceous gland, H&E, x50

Image 516: Nailbed, H&E, x20

Image 517: Central portion of nail (nailbed/matrix), H&E, x66

Image 518: Nail, at junction with skin at tip of finger (hyponychium), H&E, x66

Image 519: Pacinian corpuscle in thumb, H&E, x13

 

Nervous System (images 601 - 616 provided by Christine Hulette, M.D.)

Image 601: Cerebellum, H&E + Luxol Fast Blue stain, x66

Image 602: Cerebral cortex, H&E + Luxol Fast Blue stain, x20

Image 603: Cerebral motor cortex, H&E + Luxol Fast Blue stain, x200

Image 604: Dorsal root ganglion, H&E + Luxol Fast Blue stain, x400

Image 605: Ependyma, H&E + Luxol Fast Blue stain, x200

Image 606: Hypothalamus, H&E + Luxol Fast Blue stain x4

Image 607: Hypothalamus, H&E + Luxol Fast Blue stain x200

Image 608: Peripheral nerve, H&E, x200

Image 609: Pineal gland, H&E + Luxol Fast Blue stain, x2.5

Image 610: Pineal gland, H&E, x200

Image 611: Pons, H&E + Luxol Fast Blue stain, x2.5

Image 612: Putamen, H&E + Luxol Fast Blue stain, x66

Image 613: Sagital sinus, H&E + Luxol Fast Blue stain, x4

Image 614: Spinal cord nerve root, H&E + Luxol Fast Blue stain, x66

Image 615: Spinal cord, H&E + Luxol Fast Blue stain, x4

Image 616: White matter, H&E + Luxol Fast Blue stain, x400

Image 617: Nerve, H&E, x132. Note difference in appearance between nerve (N) and collagen (C).

 

 Urinary System

Image 701: Urethra, transitional epithelium, H&E, x132

 

Male Reproductive System

Image 801: Prostate, H&E, x10

Image 802: Prostate, H&E, x66