PTH225Duke University
PTH225
Introduction to Systemic Histology
send questions or comments to Laura P. Hale, MD. PhD. at hale0004@mc.duke.edu
The purpose of this course is to teach students how to identify various cell types in standard histologic sections and to identify a variety of normal tissues. This course will be useful to any student whose research requires examination of tissue sections. Structure/function relationships will be emphasized, using an organ system approach. The scheduled class time will include lecture and demonstration of various tissue slides by the instrutor. Beginning in 2005, the course will be taught primarily using "virtual microscopy", supplemented by examination of glass slides using a conventioanl optical microscope. In virtual microscopy, glass slides scanned at high resolution are viewed on a computer monitor. Students have the ability to select regions of intrest for higher magnification viewing and in many cases, can also change the focus. Unless arrangements are made in advance, students are expected to bring their own laptop computer to class for each class period.
Text: Histology: A Text and Atlas (4th Edition), by M.H. Ross, G.I. Kaye, W. Pawlina Lippincolt. Williams and Wilkins, Philadelphia, 2003. (Available at the Medical Center Bookstore $59)
Accessory Gastrointestinal Glands
Image 1: Serous demilunes from submandibular salivary gland, H&E, x100
Image 2: Pancreatic islet of Langerhans, glucagon immunoperoxidase stain, x66
Image 3: Pancreatic islet of Langerhans, insulin immunoperoxidase stain, x66
Image 4: Pancreatic islet of Langerhans, somatostatin immunoperoxidase stain, x66
Image 5: Mucous acini from labial (minor) salivary gland, H&E, x50
Image 6: Mixed (serous and mucous) acini from submandibular salivary gland, H&E, x100
Image 7: Exocrine pancreas, H&E, x66
Image 8: Parotid salivary gland, H&E, x100
Image 9: Serous acini from parotid salivary gland, H&E, x100
Image 10: Excretory duct from submandibular salivary gland, H&E, x50
Image 11: Submandibular salivary gland, H&E, x100
Image 12: Serous demilunes from submandibular salivary gland, H&E, x100
Image 13: Submandibular salivary gland, H&E, x100
Image 14: Pancreas, with islet of Langerhans, H&E, x66
Image 15: Liver, H&E, x33
Image 16: Pancreas, with islet of Langerhans, H&E, x66
Image 17: Pancreas, with portions of 3 islets of Langerhans, H&E, x66
Image 18: Submandibular gland, H&E, x66
Endocrine Organs (images 33-51 provided by Edward Bossen, M.D.)
Image 30: Thyroid, H&E, x66
Image 31: Thyroid, H&E, x66
Image 32: Adrenal gland, H&E, x13
Image 33: Adrenal gland, H&E, x4
Image 34: Adrenal gland, cortex and medulla, H&E, x13
Image 35: Adrenal gland, zona glomerulosa of cortex, H&E, x66
Image 36: Adrenal gland, cortex, H&E, x33
Image 37: Adrenal gland, zona reticularis of cortex, H&E, x66
Image 38: Adrenal gland, medulla, H&E, x66
Image 39: Adrenal gland, neurons present in adrenal medulla, H&E, x66
Image 40: Parathyroid gland, H&E, x13
Image 41: Parathyroid gland, chief and oxyphil cells, H&E, x400
Image 42: Parathyroid gland, chief and oxyphil cells, H&E, x400
Image 43: Pituitary gland, colloid area, H&E, x66
Image 44: Pituitary gland, prolactin immunoperoxidase stain (brown), x132
Image 45: Pituitary gland, ACTH immunoperoxidase stain (brown), x132
Image 46: Posterior pituitary (neurohypophysis), H&E, x66
Image 47: Pituitary gland, anterior and posterior portions, H&E, x66
Image 48: Anterior pituitary, basophils, acidophils, and chromophobes, H&E, x132
Image 49: Anterior pituitary, H&E, x66
Image 50: Anterior pituitary, human growth hormone (HGH) immunoperoxidase stain, x132
Image 51: Anterior pituitary, synaptophysin immunoperoxidase stain (brown), x66
Connective Tissue, Bone, Muscle
Image 60: Brown and white adipose tissue, periadrenal region, H&E, x66
Image 61: Smooth muscle, muscularis externa of colon, H&E, x132
Image 62: Brown adipose tissue, periadrenal region, H&E, x132
Image 63: White adipose tissue, periadrenal region, H&E, x132
Image 64: Smooth muscle, muscularis externa of colon. H&E, x132
Image 65: Hyaline cartilage, bronchus, H&E, x66
Image 66: Bone, vertebral column, H&E, x66
Image 67: Tendon, dense regular connective tissue, H&E, x66
Image 68: Skeletal muscle, longitudinal section, H&E, x132
Image 69: Smooth muscle, myometrium of uterus, H&E, x132
Hematopoietic and Lymphoid Systems
Image 100: Spleen, H&E, x3.3
Image 101: Spleen, periarteriolar lymphoid sheath, H&E, x33
Image 102: Adenoids, H&E, x132
Image 103: Thymus, from infant, H&E, x66
Image 104: Plasma cell, Wright stain, x400
Image 105: Bone marrow, H&E, x66
Image 106: 7 week fetus (to show location of thymus), H&E, x3.3
Image 107: Thymus, from 7 week fetus, H&E, x33
Image 108: Thymus, from 38 year old female, H&E, x16
Image 109: Thymus, from 42 year old male, H&E, x3.3
Image110: High endothelial (post-capillary) venule, from adenoid tissue, H&E, x132
Image 111: Adenoids, H&E, x66
Image 112: Lymph node, H&E, x16
Image 113: Spleen, red pulp, H&E, x66
Image 114: Tonsil, H&E, x33
Image 115: High endothelial (post-capillary) venules, from tonsil, H&E, x132
Image 116: Mast cells, transmission electron microscopy
Image 117: Mast cell, safranin stain, x400
Image 118: Mast cell, scanning (left) and transmission (right) electron microscopy
Image 119: Mast cell cluster, toluidine blue stain, x400
Image 120: Mast cell cluster, toluidine blue stain, x400
Image 121: Mast cell, safranin stain, x400
Image 122: Mast cell, toluidine blue, x400
Image 123: Peyer's patch, ileum, H&E, x33
Gastrointestinal System
Image 200: Duodenum: mucosa, muscularis mucosae, and submucosa, H&E, x66
Image 201: Colon, H&E, x66
Image 202: Myenteric plexus of colon, H&E, x132
Image 203: Muscularis externa of colon, H&E, x13
Image 204: Myenteric plexus of colon, H&E, x132
Image 205: Stratified squamous epithelium, esophagus, H&E, x33
Image 206: Stomach, gastrin immunoperoxidase stain (brown), x33
Image 207: Stomach, simple columnar epithelium, H&E, x50
Image 208: Ileum, H&E, x3
Image 209: Ileum, H&E, x132. Arrows point out brush border on epithelial cells.
Image 210: Colon: mucosa, submucosa, muscularis externa, serosa, H&E, x4
Image 211: Colon, H&E, x33
Image 212: Ileum, H&E, x33
Image 213: Appendix, H&E, x3
Image 214: Appendix, H&E, x3
Image 215: Oral mucosa, H&E, x33. Note salivary gland underlying non-keratinized stratified squamous epithelium.
Female Reproductive System
Image 300: Breast, H&E, x66
Image 301: Breast, H&E, x4
Image 302: Placenta, chorionic villus, H&E, x132
Image 303: Endometrium, late secretory (day 24), H&E, x13/ x33
Image 304: Endometrium, proliferative phase, H&E, x13
Image 305: Endometrium, secretory phase, H&E, x13
Image 306: Fallopian tube, ciliated columnar epithelium, H&E, x400
Image 307: Lactating breast, H&E, x13
Image 308: Lactating breast, H&E, x66
Image 309: Cervix, stratified squamous epithelium, H&E, x50
Image 310: Endocervix, ciliated columnar epithelium, H&E, x50
Image 311: Breast, H&E, x13
Image 312: Chorioamnionic membrane, H&E, x33
Image 313: Placenta, chorionic villi, H&E, x33
Image 314: Placenta, chorionic villi, H&E, x66
Image 315: Fallopian tube, showing variation in size and complexity along tube length, H&E, x1
Image 316: Ovary, simple cuboidal germinal epithelium, H&E, x132
Image 317: Ovary, germinal epithelium and tunica albuginea, H&E, x66
Image 318: Ovary, cortical stroma with multiple primordial follicles, H&E, x33
Image 319: Ovary, medulla containing multiple blood vessels, H&E, x33
Image 320: Fallopian tube, columnar epithelium, H&E, x132
Image 321: Fallopian tube, H&E, x4. Note vessels in mesosalpinx at left.
Image 322: Fallopian tube, H&E, x13
Image 323: Mesothelium overlying fallopian tube, H&E, x66
Cardiovascular System
Image 400: Heart, H&E, x130
Image 401: Artery and vein, periadrenal region, H&E, x66
Image 402: Artery, H&E, x13
Image 403: Artery, H&E, x25
Image 404: Artery, H&E, x100
Image 405: Heart, H&E, x33
Image 406: Artery, H&E, x33. Arrow points to internal elastic lamina.
Image 407: Vein, H&E, x33. N denotes nerve adjacent to vessel.
Respiratory System
Image 450: Bronchial wall, cartilage and mucous glands, H&E, x33
Image 451: Bronchial epithelium, H&E, x132
Image 452: Bronchial epithelium, H&E, x132
Image 453: Bronchial epithelium. H&E, x132
Skin and Associated Structures (images 500 - 518 provided by Christopher Shea, M.D.)
Image 500: Epidermis, H&E, x100
Image 501: Epidermis, H&E, x200
Image 502: Epidermis (note prominent melanocyte), H&E, x200
Image 503: Skin, showing papillary and reticular dermis and vascular plexus, H&E, x20
Image 504: Dermal blood vessels and nerve, H&E, x200
Image 505: Dermal artery, H&E, x50
Image 506: Dermal vein, H&E, x50
Image 507: Artery and nerve, at junction of reticular dermis and subcutaneous adipose tissue, H&E, x40
Image 508: Skin, showing epidermis, dermis, and eccrine unit. H&E, x5
Image 509: Eccrine gland, H&E, x200
Image 510: Epidermis, showing eccrine duct exiting to skin surface (acrosyringium), H&E, x100
Image 511: Base of hair follicle (bulb), H&E, x50
Image 512: Middle of hair follicle (isthmus), including sebaceous gland. H&E, x50
Image 513: Upper portion of hair follicle (infundibulum), H&E, x50
Image 514: Smooth muscle associated with hair (arrector pili muscle), H&E, x200
Image 515: Sebaceous gland, H&E, x50
Image 516: Nailbed, H&E, x20
Image 517: Central portion of nail (nailbed/matrix), H&E, x66
Image 518: Nail, at junction with skin at tip of finger (hyponychium), H&E, x66
Image 519: Pacinian corpuscle in thumb, H&E, x13
Nervous System (images 601 - 616 provided by Christine Hulette, M.D.)
Image 601: Cerebellum, H&E + Luxol Fast Blue stain, x66
Image 602: Cerebral cortex, H&E + Luxol Fast Blue stain, x20
Image 603: Cerebral motor cortex, H&E + Luxol Fast Blue stain, x200
Image 604: Dorsal root ganglion, H&E + Luxol Fast Blue stain, x400
Image 605: Ependyma, H&E + Luxol Fast Blue stain, x200
Image 606: Hypothalamus, H&E + Luxol Fast Blue stain x4
Image 607: Hypothalamus, H&E + Luxol Fast Blue stain x200
Image 608: Peripheral nerve, H&E, x200
Image 609: Pineal gland, H&E + Luxol Fast Blue stain, x2.5
Image 610: Pineal gland, H&E, x200
Image 611: Pons, H&E + Luxol Fast Blue stain, x2.5
Image 612: Putamen, H&E + Luxol Fast Blue stain, x66
Image 613: Sagital sinus, H&E + Luxol Fast Blue stain, x4
Image 614: Spinal cord nerve root, H&E + Luxol Fast Blue stain, x66
Image 615: Spinal cord, H&E + Luxol Fast Blue stain, x4
Image 616: White matter, H&E + Luxol Fast Blue stain, x400
Image 617: Nerve, H&E, x132. Note difference in appearance between nerve (N) and collagen (C).
Urinary System
Image 701: Urethra, transitional epithelium, H&E, x132
Male Reproductive System
Image 801: Prostate, H&E, x10
Image 802: Prostate, H&E, x66
